Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca2+ Ionophore

نویسندگان

  • Abdolhossein Shahverdi
  • Mojtaba Rezazadeh Valojerdi
  • Saeid Kazemi Ashtiani
چکیده مقاله:

The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or without CaI-treated sperms. Results: Piezo-ICSI demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional ICSI: 42%, P<0.001) and a cleavage rate of 73% (conventional ICSI: 58%, P<0.05). When the Piezo-ICSI procedure was performed with CaI-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, P<0.05). However, the fertilization rate did not change significantly (64% vs. 56%). Conclusion: The Piezo-ICSI accompanies with CaI-treated sperms is more efficient than the conventional ICSI method for fertilizing and thus obtaining more mouse embryos.

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منابع مشابه

comparison of embryo development between intracytoplasmic and piezo-assisted sperm injection after treating mouse sperms by ca2+ ionophore

the purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (icsi) and piezo-assisted sperm injection after pretreatment with calcium ionophore (cai) on the mouse embryo development. methods: in this study, the conventional icsi and piezo-icsi procedures were used. the efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...

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Comparison of embryo development between intracytoplasmic and Piezo-assisted sperm injection after treating mouse sperms by Ca2+ ionophore.

BACKGROUND The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. METHODS In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized w...

متن کامل

Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca Ionophore

Background: The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized w...

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Piezo-actuated Mouse ICSI (Intracytoplasmic sperm injection)

Intracytoplasmic sperm injection (ICSI) is not only used in human assisted reproduction (ART), but it is also widely employed for veterinary in vitro fertilization during rare species preservation, germ-line rescue of transgenic animals or for any veterinary assisted conception. Piezo-assisted ICSI, which was first described in 1995 [1], can be employed for assisted conception in animals in whi...

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Comparison between intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection in oligo-astheno-teratozoospermia patients

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عنوان ژورنال

دوره 11  شماره 4

صفحات  245- 250

تاریخ انتشار 2007-10

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